한국법과학회지, Vol.22 no.2 (2021)
pp.41~49
- 위생용품이 체액의 DNA에 미치는 영향 -
Many DNA samples are exposed to various environments at crime scenes and mixed with other substances that adversely affect STR profiling. In this study, DNA quantification and STR profiling were performed up to 40 weeks with body wash or hand sanitizer mixed with saliva, blood, and semen at a final concentration of 1%. In addition, serological preliminary tests such as SALIgAE, LMG, and AP and immunological confirmatory tests using RSID kits were conducted to examine the effect of body wash and hand sanitizer on three body fluids. Body wash containing surfactants showed more serious effect on DNA quantity and STR quality than hand sanitizer containing ethanol. Saliva was immediately decomposed after mixing hygiene products, making it impossible to analyze STR, however blood and semen produced full STR profiles after 40 weeks of mixing. As a result, saliva was very vulnerable to hygiene products, but blood and semen were not affected until 40 weeks after mixing. In addition, the discrepancy between serological test and STR profiling in blood and semen samples showed that STR analysis should be performed even if the serological test was negative.