Gasoline is highly flammable substance which also shows chronic toxicity when inhaled. Accidental poisoning cases caused by hydrocarbon fuel occur continuously in modern times, therefore the demand of standardized method and threshold in gasoline poisoning cases are increasing in the forensic science field. In this study, analysis conditions and results for the detection of each components in gasoline from fatal poisoning case using solid-phase microextraction and solvent extraction are presented. Specific composition distribution patterns of autopsy samples and field samples are estimated from detection rate and score of each components. From field samples, higher alkanes were mainly found, while autopsy samples had lower alkanes which have relatively high penetrating ratio into body. Besides, aromatic compounds showed relatively high loss rate in both samples, because of their high volatility.