The crackdown on the drunk driving is continuously enforced and the drivers might not agree with the result of the blood alcohol test results. Especially the test result could be controversial for the blood sampled by using disinfection ethanol swab, without using the sterile liquid at the blood sampling kit. In this study, the effect of the disinfection ethanol swab on the concentration when sampled by syringe under ethanol remaining condition through SPME-GC/MS analysis with the internal standard of isotope substituted ethanol(CD3CD2OH). The sample taken through the dried pig skin didn’t show meaningful value of ethanol but the result of ethanol residual case showed the ethanol concentration from the value of quantification limit ∼ 0.003% with the average value of about 0.001%. The quantification limit of this method was about 0.0005% and the linearity of the calibration curve showed the r2 of 0.9999 or more which was plotted by the area response ratio between the ion of m/z 45 for ethanol and m/z 49 for internal standard.